Fc Binding and Function Assays
Abzena offers a complete solution to support the characterisation of the Fc-mediated activity of your antibody or ADC product.
Fc-mediated effector functions may play a role in the mechanism of action of an antibody or an ADC and have an impact on the product safety and efficacy. For these reasons, both EMEA and FDA guidelines recommend to analyse Fc functions in detail, including binding to Fc receptors and Fc-mediated cytotoxicity such as ADCC and CDC.
Right: Simplified schematic overview of mAb-induced effector mechanisms (Nuray Gül, and Marjolein van Egmond Cancer Res 2015;75:5008-5013) ©2015 by American Association for Cancer Research
Measure the binding kinetics of your antibody or ADC product to Fc-gamma and neonatal Fc receptors. Read more >>>
Assess whether your antibody or ADC triggers immune effector cell-mediated lysis of antigen expressing target cells. Read more >>>
Evaluate the ability of your antibody or ADC product to recruit and activate human complement, leading to the lysis of antigen expressing target cells. Read more >>>
Determine whether your antibody or ADC can fix C1q, the first component of the classical complement pathway. Read more >>>