Abzena’s phage display technology, using naïve and immune libraries (from a variety of species, including llamas), can be used to identify lead candidates against a vast range of targets, including:
|TNF superfamily receptors and ligands||Blood proteins|
|Brain neurology targets||Tumour biomarkers|
|Cytokines and receptors||Immunoglobulins|
|T cell activators, ligands and receptors||Checkpoint modulators|
|Non protein targets|
In phage display technology, antibody fragments (Fabs) are fused to the ﬁlamentous bacteriophage M13 gene III coat proteins, thus allowing billions of phages to be produced that each display on their surface an antibody fragment with a unique speciﬁcity. These highly diverse phage populations can then be presented to the desired target and, through a process of selection, ampliﬁcation and re-selection (panning), antibody fragments can be isolated that have high speciﬁcity for the antigen.
Due to the ﬂexibility of the phage display-based selection process, this technology enables the identiﬁcation of antibody fragments with very speciﬁc characteristics, representing an alternative to the classical hybridoma technology.
By reducing the concentration of antigen and the use of stringent conditions during panning, Fabs can be selected that have high aﬃnity/potency and are stable. Selection for function, for example antagonism, agonism, internalization and epitope speciﬁcity are also possible.
This technology has been used in projects such as generating mAbs against diﬀerent domains of llama IgG1 and generating capture and detection mAbs for cancer and heart biomarkers.
Libraries used in phage display include:
Llama VHH Naïve Library
The 1.5 billion llama VHH naïve library was generated using biological samples from ten diﬀerent animals and can be used to select binders against virtually any antigen. Naïve libraries are ideal for use when immunisations are not appropriate for reasons such as limits to antigen availability, antigen toxicity or where epitopes are immunodominant.
Llama VHH single domain antibodies are stable, resistant to low pH, have high thermostability and have increased hydrophobicity compared to VHHs engineered from other species.
Human Fab Naïve Library
The human library contains over 10 billion Fabs ready for the discovery binders to your target.
The library comprises Fabs rather than single chain variable fragments (scFvs) as is the case for some other human libraries. Abzena can undertake the conversion of selected Fabs to IgG and, compared to scFvs, Fabs are much less prone to aggregation issues and loss of activity when re-formatting, thus improving the chances of delivering the desired target speciﬁcities and properties.
Working with Abzena
Abzena’s discovery services are tailored for each project to ensure that the objectives are met or exceeded. Experienced project teams are assigned to each study focusing on progressing projects through to results in the minimum amount of time. Our clients widely regard us as professional and attentive partners who deliver quality results.
To get more information, a quote or to schedule a teleconference please contact us.
Abzena discovery services are offered in collaboration with: