Antibody-Dependent Cell-mediated Cytotoxicity (ADCC)
ADCC is an immune response leading to lysis of antibody-coated target cells by immune effector cells. ADCC is triggered by the interaction between the Fc portion of an antibody and Fc-gamma receptors expressed on immune cells such as NK cells. ADCC assays can be performed at Abzena using human PBMCs or NK cells as effector cells. Typically, antibody-coated target cells are incubated in the presence of effector cells to induce cell lysis. Dead cells are then quantified either using a fluorophore release or label-free assay.
Working with Abzena
Abzena undertakes bioassays on behalf of clients as standalone projects or as part of larger development projects. We focus on developing the correct bioassay to help you understand your molecule.
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CDC Assay Ramos Target Cells
Trastuzumab and Kadcyla® induce dose-dependent lysis of Her2-expressing SK-BR-3 cells by PBMCs when co-cultured in a 40:1 Effector:Target ratio.