Binding & Competition – Abzena

Binding and Competition Assays

Lead identification and subsequent lead characterisation often rely on the in vitro assessment of protein or antibody binding to their targets. Abzena can assess binding to both targets expressed on cells and to soluble proteins. We offer a range of assays that can be tailored to your specific project.

Cell binding can be measured by flow cytometry or fluorescence microscopy, whereas ELISA, HTRF and SPR are the methods of choice to investigate binding to soluble proteins. Both direct and competitive binding assays are typically performed. Full kinetic analysis can be performed using our Biacore T200.

biacore

Multicycle kinetics of two antibodies using the Biacore T200

flow-cytometry

Assessment of anti-PSMA mAb binding to a prostate cancer cell line by flow cytometry using a fluorescently labelled secondary antibody as detection reagent.

Working with Abzena

Abzena undertakes bioassays on behalf of clients as standalone projects or as part of larger development projects. We focus on developing the correct bioassay to help you understand your molecule.

To get more information, a quote or to schedule a teleconference please contact us.

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